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The highest n-alkane degradation occurred in the B50 and B10 blends. The highest biodegradation rates of the C16:0, C18:0, C18:1 cis and C18:2 FAMEs (25.5, 17.5%, 34.5%, and 27.7%, respectively) occurred in the B5 blend. The abundance of bacteria with the alkB gene found in the blends was one or two orders of magnitude higher than that of the total bacteria estimated.
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The fungal population formed at the oil-water interface was larger than the bacterial population, in all treatments. Microorganism abundance was estimated by the amplification of 16S rRNA and 18S rRNA genes using qPCR. After 60 days of incubation, B100 and B50 had the highest biomass production at the oil-water interface. The effect of contamination on the biodegradation of fatty acid methyl esters (FAMEs) and n-alkanes was assessed by GC/qMS. Fungi and bacteria were quantified by qPCR. During the simulation, and after contamination with inoculum, microbial growth was monitored through biomass formation. In this study, the storage of pure diesel (B0), diesel and biodiesel blends (B5, B10, and B50), and pure biodiesel (B100) was simulated. Understanding the changes in microbial community structure and its impact during the storage of diesel and biodiesel is critical to prevent damage to the system and to ensure a good quality product to consumers.